نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترای گروه مکانیک بیوسیستم، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
2 گروه مکانیک بیوسیستم، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران
3 گروه فیزیک اتمی و مولکولی، دانشکده علوم پایه دانشگاه مازندران، بابلسر، ایران
4 گروه بیولوژی مولکولی و گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده بهداشت، دانشگاه علوم پزشکی، تهران، تهران، ایران
چکیده
کپک سبز و آبی عوامل محدودکننده دوره انبارداری بسیاری از میوهها و سبزیها از جمله مرکبات میباشد. در این تحقیق اثر پلاسمای سرد فشار اتمسفری تولیدشده توسط تخلیه اسپارک گذرا جریان مستقیم در یک راکتور بسته بر روی سوسپانسیون قارچ پنیسیلیوم دیجیتاتوم بررسی شد. سوسپانسیون با غلظتهای اولیه متفاوت شامل: صفر، پنج صدم، یک دهم و پنج دهم درصد پراکسیدهیدروژن در زمانهای مختلف معرض پلاسما 2.5، 5، 10 و 15 دقیقه تحت پلاسمادهی قرار داده شدند. نتایج نشان داد که با افزایش زمان پلاسمادهی و نیز غلظت پراکسیدهیدروژن محلول اثر غیرفعالسازی اسپورهای قارچ افزایش یافت. در غلظتهای یک و پنج دهم درصد میزان توقف کامل اسپورها در زمان 15 دقیقه پلاسمادهی مشاهده شد. پلاسمادهی توانست سبب افزایش اکسیداسیون محلول شده و در نتیجه منجر به مرگ سلولی بیشتر اسپورها شد. بنابراین استفاده از پلاسمای سرد موجب میشود که استفاده از محلولهای با غلظت بالای پراکسیدهیدروژن در هنگام شستشوی میوهها و سبزیها بهمنظور حفظ خواص پس از برداشت آنها در مقیاس صنعتی اجتناب شود.
کلیدواژهها
موضوعات
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